Bond-force measurements with Streptavidin Beads (Seradyn)

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Full video
Probe: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Seradyn (diameter: 0,779µm; Streptavidin) 50µg/ml
Video : 3-10_6-7_B_Oben
Area : 48,1 x 48,1µm (150x150+414+153)
  • The current given in the video flows through the wide left conducting line.
  • A bead is ruptured between 23 and 24sec. The current increases from 19 to 21mA (collision?).
   

Full video
Probe: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Seradyn (diameter: 0,779µm; Streptavidin) 50µg/ml
Video : 3-10_6-7_B_Mitte
Area : 48,1 x 48,1µm (150x150+422+483)
  • The current given in the video flows through the wide left conducting line.
  • A bead is ruptured between 39 and 40sec. The current increases from 34 to 37mA.
   

Full video
Probe: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Seradyn (diameter: 0,779µm; Streptavidin) 50µg/ml
Video : 3-10_6-7_C
Area : 48,1 x 48,1µm (150x150+406+407)
  • The current given in the video flows through the wide left conducting line.
  • A bead is ruptured between 46 and 47sec. The current increases from 40 to 45mA.