Bond-force measurements with Avidin Beads (Micromod)

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Full video 		Sample: S6_9
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 16-21_17-20_A
Area : 48,1 x 48,1µm (150x150+334+873)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 115 and 116secs at a constant current of 82mA.
   

Full video 		Sample: S6_9
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 16-21_17-20_C
Area : 64,2 x 64,2µm (200x200+430+129)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 68 and 69sec. The current increases from 68 to 69mA.
  • Another bead agglutination is ruptured between 72 and 73sec. The current increases from 74 to 76mA (collision?).
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_B_oben
Area : 48,1 x 48,1µm (150x150+447+292)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 66 and 67sec. The current increases from 8 to 9mA.
  • Another bead is ruptured between 70 and 71sec. The current increases from 12 to 15mA.
  • Another bead is ruptured between 73 and 74sec. The current increases from 14 to 15mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_B_unten
Area : 48,1 x 64,2µm (150x200+461+741)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 87 and 88sec. The current increases from 24 to 25mA.
  • Another bead is ruptured between 158 and 159sec at a constant current of 87mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_C_oben
Area : 48,1 x 48,1µm (150x150+450+110)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 87 and 89sec. The current increases from 48 to 54mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_C_mitte
Area : 64,2 x 64,2µm (200x200+445+317)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 24 and 26sec. The current increases from 6 to 9mA.
  • A bead agglutination is ruptured between 40 and 45sec. The current increases from 15 to 17mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_C_unten
Area : 64,2 x 64,2µm (200x200+445+508)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 91 and 92sec. The current increases from 57 to 58mA.
  • A part of a bead agglutination is ruptured between 96 and 98sec. The current increases from 60 to 62mA.
  • A bead is ruptured between 99 and 100sec (the rest of a bead agglutination). The current increases from 64 to 63mA.
  • A part of a bead agglutinationis ruptured between 148 and 150sec. The current increases from 0 to 86mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_D_oben
Area : 48,1 x 56,2µm (150x175+452+28)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 15 and 17sec at a constant current of 10mA.
  • A bead agglutination is ruptured between 25 and 27sec. The current increases from 16 to 18mA.
  • A bead is ruptured between 92 and 93sec at a constant current of 59mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_D_mitte1
Area : 48,1 x 48,1µm (150x150+464+213)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 109 and 110sec. The current increases from 76 to 77mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_D_mitte2
Area : 48,1 x 48,1µm (150x150+476+533)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 24 and 25sec. The current increases from 15 to 16mA.
  • A bead is ruptured between 25 and 27sec. The current increases from 16 to 18mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_D_unten
Area : 48,1 x 48,1µm (150x150+469+753)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 19 and 20sec. The current increases from 10 to 12mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_E_mitte
Area : 48,1 x 48,1µm (150x150+473+430)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 58 and 60sec. The current increases from 22 to 23mA.
  • A bead agglutination is ruptured between 124 and 125sec at a constant current of 63mA.
   

Full video 		Sample: S6_10
Surface : Silan, Biotin 1/10 diluted (1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-21_16-20_E_unten
Area : 48,1 x 48,1µm (150x150+478+621)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 84 and 86sec at a constant current of 41mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-22_18-19_A_oben
Area : 48,1 x 64,2µm (150x200+477+156)
  • The current given in the video flows through the right wide conducting line.
  • A bead agglutination is ruptured between 135 and 138sec at a constant current of 13mA.
  • A bead is ruptured between 231 and 232sec. The current increases from 62 to 66mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-22_18-19_A_mitte
Area : 48,1 x 48,1µm (150x150+488+474)
  • The current given in the video flows through the right wide conducting line.
  • A bead agglutination is ruptured between 203 and 204sec at a constant current of 43mA.
  • A bead is ruptured between 231 and 232sec The current increases from 62 to 66mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Micromod (diameter: 1,5µm; Avidin) (1/200: 50µg/ml)
Video : 15-22_18-19_A_unten
Area : 48,1 x 48,1µm (150x150+496+733)
  • The current given in the video flows through the right wide conducting line.
  • A bead is slowly ruptured between 127 and 136sec. The current increases from 7 to 13mA.


Michael Schilling