Bond-force measurements with Streptavidin Beads (Chemagen)

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Full video 		Sample: Sulfid6K
Surface : Silan, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 2-11_1-12_B
Area : 48,1 x 48,1 µm (150x150+486+386)
  • The current given in the video flows through the left wide conducting line.
  • A bead is ruptured between 66 and 67sec. The current increases from 39 to 58mA.
   

Full video 		Sample: Sulfid6L
Surface : Silan, Biotin 1/1000 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 17-20_16-21_A
Area : 64,2 x 64,2µm (200x200+318+358)
  • The current given in the video flows through the wide conducting line (second from the right).
  • A bead agglutination is ruptured between 36 and 37sec. The current increases from 40 to 59mA.
   

Full video 		Sample: Sulfid6L
Surface : Silan, Biotin 1/10 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_B_Unten
Area : 64,2 x 64,2µm (200x200+311+791)
  • The current given in the video flows through the wide right conducting line.
  • A bead is ruptured between 75 and 76sec. The current increases from 38 to 60mA.
   

Full video 		Sample: Sulfid6L
Surface : Silan, Biotin 1/10 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_B_Mitte
Area : 64,2 x 64,2µm (200x200+300+446)
  • The current given in the video flows through the wide right conducting line.
  • A bead is partly ruptured between 70 to 71sec. The current increases from 20 to 40mA.
  • The bead is fully ruptured between 89 and 90sec. The current increases from 60 to 79mA.
   

Full video 		Sample: Sulfid6L
Surface : Silan, Biotin 1/10 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_C
Area : 64,2 x 64,2µm (200x200+297)
  • The current given in the video flows through the wide right conducting line.
  • A bead is ruptured between 74 and 76sec at a constant current of 98mA. A collision with another bead seems to trigger the rupture event.
   

Full video 		Sample: Sulfid6M
Surface : Silan, Biotin 1/1000 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_G
Area : 64,2 x 64,2µm (200x200+316+359)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is partly ruptured between 37 and 40sec. The current increases from 17mA to 20mA.
   

Full video 		Sample: Sulfid6M
Surface : Silan, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 13-24_14-23_A
Area : 64,2 x 64,2µm (200x200+301+370)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is ruptured between 58 and 59sec. The current is nearly constant at 67mA. The rupture event seems to be triggered by a collision with another bead.
   

Full video 		Sample: Sulfid6M
Surface : Silan, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 13-24_14-23_B
Area : 64,2 x 64,2µm (200x200+432+579)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is ruptured between 24 and 25sec. The current increases from 22-25mA.
   

Full video 		Sample: Sulfid6M
Surface : Silan, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 13-24_14-23_C
Area : 48,1 x 48,1µm (150x150+443+108)
  • The current given in the video flows through the wide conducting line on the left.
  • A part of a bead agglutination is ruptured between 53 and 55sec ab. The current increases from 19 to 21mA.
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 13-24_14-23_A
Area : 48,1 x 48,1µm (150x150+437+508)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is ruptured between 31 and 32sec. The current increases from 25 to 27mA.
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 13-24_14-23_B
Area : 48,1 x 48,1µm (150x150+396+470)
  • The current given in the video flows through the wide conducting line on the left.
  • A first bead is ruptured between 50 and 51sec. The current increases from 73 to 74mA.
  • A second bead is ruptured between 55 and 55sec.The current increases from 79 to 81mA.
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_A
Area : 48,1 x 48,1µm (150x150+447+95)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is slowly ruptured between 42 and 44sec. The current increases from 27 to 32mA (collision?).
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_B
Area : 48,1 x 48,1µm (150x150+433+480)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is ruptured between 30 and 31sec. The current increases from 15 to 18mA.
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_B
Area : 48,1 x 48,1µm (150x150+424)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is slowly ruptured between 41 and 53sec. The current increases from 29 to 46mA.
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_C
Area : 48,1 x 48,1µm (150x150+374+55)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is ruptured between 34 and 35sec. The current increases from 27 to 29mA (collision!).
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_C
Area : 48,1 x 48,1µm (150x150+384+242)
  • The current given in the video flows through the wide conducting line on the left.
  • A bead is ruptured between 79 and 81sec. The current increases from 63 to 67mA.
   

Full video 		Sample: S6_8
Surface : Epoxy, Biotin 1/100 diluted
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 16-21_17-20_D
Area : 48,1 x 160,5µm (150x500+394+105)
  • The current given in the video flows through the wide conducting line on the left.
  • 6 beads are ruptured between 18 and 19sec at a current of 11mA.
  • Another bead is ruptured between 19 and 20sec at a current of 12mA.
  • Another bead is ruptured between 25 and 26sec at a current of 17mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_B_unten
Area : 48,1 x 48,1µm (150x150+492+816)
  • The current given in the video flows through the wide right conducting line.
  • A bead is ruptured between 43 and 44sec. The current increases from 15 to 16mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_C_oben
Area : 48,1 x 128,4µm (150x400+473+17)
  • The current given in the video flows through the wide right conducting line.
  • A bead is ruptured between 29 and 30sec. The current increases from 10 to 11mA.
  • 3 beads are ruptured between 30 and 32sec (2 at the top and 1 at the bottom). The current increases from 11 to 12mA.
  • A bead is ruptured between 35 and 36sec at a constant current of 12mA.
  • A bead is ruptured between 39 and 40sec at a constant current of 12mA.
  • A bead is ruptured between 40 and 42sec at a constant current of 12mA.
  • A bead is ruptured between 49 and 50sec at a constant current of 19mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_C_mitte
Area : 48,1 x 48,1µm (150x150+483+457)
  • The current given in the video flows through the wide right conducting line.
  • A bead is ruptured between 29 and 30sec. The current increases from 10 to 11mA.
   

Full video 		Sample: S6_11
Surface : Silan, Biotin 1/100 diluted (0,1µM)
Beads : Chemagen (diameter: 1µm; Streptavidin) 50µg/ml
Video : 4-9_5-8_C_unten
Area : 48,1 x 48,1µm (150x150+496+827)
  • The current given in the video flows through the wide right conducting line.
  • A bead is ruptured between 99 and 100sec. The current increases from 68 to 69mA.


Michael Schilling